![]() ![]() In early 2000 we have established a “minimal” experimental system that maintains transcription through various defined mono- and polynucleosomes by RNA polymerase II. The questions are: Can the differences in affinity be explained by the chromatin context of the binding sites? What changes in chromatin structure are involved in p53-DNA interaction? ![]() We are trying to understand the mechanisms of interaction of p53 with its binding sites in chromatin. It has an apparent high affinity to the response elements regulating cell cycle arrest genes (CCA-sites) and a lower affinity to the sites associated with apoptosis (Apo-sites) in vivo. Tumor suppressor p53 is a sequence-specific DNA-binding protein, possibly a pioneering factor involved in gene regulation. Our research focuses on the following questions: What changes in chromatin structure are involved in the reorganization? How do various anti-cancer drugs affect this process? What are the mechanisms of trapping of the histone chaperones in chromatin? Recently we have studied the mechanisms of FACT- and PARP1-dependent chromatin reorganization. Importantly, both hFACT & hPARP1 are involved in carcinogenesis and are important targets for anti-cancer drugs. The mechanisms of gene regulation over a distance (by enhancers & insulators).įACT and PARP1 are histone chaperones that can increase chromatin accessibility to various proteins during initiation and elongation of transcription. The mechanisms and regulation of transcript elongation through chromatin by RNA polymerase II, and 4. Mechanisms of interaction of sequence-specific regulator proteins (e.g. Mechanisms of histone chaperones FACT and PARP1 action during transcription and its regulation. Currently our efforts are focused in the following primary directions: 1. This goal will be achieved using a combination of molecular genetics, genomics, biochemical, single-particle, structural and computational modeling approaches. The major research goal in my laboratory is to understand the molecular mechanisms and regulation of the vital process of eukaryotic transcription in chromatin, and the role of the factors involved in cancer development and human aging (e.g. Regulation of gene expression occurs primarily at the initial step (transcription) and involves DNA sequences, protein factors and dynamic changes in structure of DNA-protein complexes (chromatin). Although his own playing began to falter a bit during this time, Ferguson continued to perform for audiences the world over until his passing in 2006.Development and functioning of higher organisms critically depends on properly regulated gene expression. The latter of these ultimately became the band Ferguson was associated with for most of the rest of his life. The 1980s and 1990s saw Ferguson downsize his big band, first to a jazz-rock fusion septet called High Voltage, then to a little big band called Big Bop Nouveau. This new band featured a much more commercial sound than his previous outlets, oftentimes covering pop tunes such as Jimmy Webb's "MacArthur Park" and "Gonna Fly Now," the theme from the movie "Rocky." Although this period receives a level of scorn from more traditional jazz fans, it undeniably counts as the most popularly successful period of Ferguson's career. This band served as a launching pad for the careers of many highly notable musicians including tenor saxophonists Wayne Shorter and Joe Farrell, trombonist/arranger Slide Hampton, trumpeters Don Ellis and Bill Chase, and pianists Joe Zawinul and Jaki Byard.Īfter relocating to India and then England in the mid-1960s, Ferguson returned to the United States in 1971 with a new band. Kenton's stable of arrangers quickly found ways to make use of Ferguson's unprecedented power and control in the trumpet's extreme upper register, quickly making him a highly popular figure in the jazz world.Īfter spending several years working in the Los Angeles studio scene, Ferguson moved to New York in 1956 to form his "Birdland Dream Band." Although the band was not particularly long-lived, Ferguson took the core of this band on the road for the next decade as his primary musical outlet. His talents drew the attention of several American bandleaders during this time, ultimately earning him an invitation to join the Stan Kenton Orchestra in 1949. Taking to his new instrument extremely quickly, he was already performing professionally in his early teens. A gifted multi-instrumentalist, highly capable improviser, and renowned bandleader, his 50+ year career was filled with highlights that extended beyond mere showmanship.īorn in Montreal in 1928, Ferguson initially showed traits of being a child prodigy on violin before switching to the trumpet at age 9. Thrilling trumpet players all over the world with his high note acrobatics, Maynard Ferguson was much more than just a flashy performer. ![]()
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